The lentiviral vector is a virus vector system modified on the basis of HIV-1, which can effectively integrate foreign genes or foreign shRNA into the host chromosome, thus achieving the effect of persistent expression of the target sequence. LVV has become one of the commonly used vector forms to express foreign genes or foreign shRNA, and is getting more and more widely used. Cell Valley can provide lentiviral vector customization services for a variety of tumor targets, such as BCMA, CD19, CD38, CD22, EGFR/EGFRVIIII, etc.

LVV is a kind of viral vector modified from human immunodeficiency virus (HIV). It is a kind of retrovirus. The genome is RNA. Its toxic gene has been removed and replaced by exogenous target gene. It belongs to pseudovirus, which can integrate exogenous gene into the genome to achieve stable expression, and has the characteristics of infecting cells in mitotic and non-mitotic stages.

After the LVV genome enters the cell, it is reversely transcribed into DNA in the cytoplasm to form a DNA pre-integration complex. After entering the nucleus, DNA is integrated into the cell genome. The integrated DNA is transcribed into mRNA and returned to the cytoplasm to express the target protein or produce small RNA. The lentivirus-mediated gene expression or microRNA interference is persistent and stable, and divides with the cell genome division (Fig. 1).